Department of Life Sciences and Systems Biology

Research products

Selected research products

Catucci G., Ciaramella A., Di Nardo G., Zhang C., Castrignanò S., Gilardi G. (2022) Molecular Lego of Human Cytochrome P450: The Key Role of Heme Domain Flexibility for the Activity of the Chimeric Proteins. International Journal of Molecular Sciences 23: 3618. DOI: 10.3390/ijms23073618

Di Nardo G., Di Venere A., Zhang C., Nicolai E., Castrignanò S., Di Paola L., Gilardi G.4, Mei G. (2021) Polymorphism on human aromatase affects protein dynamics and substrate binding: spectroscopic evidence. Biology Direct 16(1): 8. DOI: 10.1186/s13062-021-00292-9

Panicco P.*, Castrignanò S.*, Sadeghi S.J., Di Nardo G., Gilardi G. (2021) Engineered human CYP2C9 and its main polymorphic variants for bioelectrochemical measurements of catalytic response. Bioelectrochemistry 138: 107729. DOI: 10.1016/j.bioelechem.2020.107729. *PP and SC equally contributed to the work.

Castrignanò S., Di Nardo G., Sadeghi S.J., Gilardi G. (2018) Influence of inter-domain dynamics and surrounding environment flexibility on the direct electrochemistry and electrocatalysis of self-sufficient cytochrome P450 3A4-BMR chimeras. Journal of Inorganic Biochemistry 188: 9-17. DOI: 10.1016/j.jinorgbio.2018.08.003.

Castrignanò S.*, D'Avino S.*, Di Nardo G., Catucci G., Sadeghi S.J., Gilardi G. (2018) Modulation of the interaction between human P450 3A4 and B. megaterium reductase via engineered loops. Biochimica et Biophysica Acta - Proteins and Proteomics 1866 (1): 116-125. DOI: 0.1016/j.bbapap.2017.07.009. *SC and SD equally contributed to the work.

Gao C., Catucci G., Castrignanò S., Gilardi G., Sadeghi S.J. (2017) Inactivation mechanism of N61S mutant of human FMO3 towards trimethylamine. Scientific Reports 7 (1): 14668. DOI: 10.1038/s41598-017-15224-9.

Degregorio D., D'Avino S., Castrignanò S., Di Nardo G., Sadeghi S.J., Catucci G., Gilardi G. (2017) Human cytochrome P450 3A4 as a biocatalyst: Effects of the engineered linker in modulation of coupling efficiency in 3A4-BMR chimeras. Frontiers in Pharmacology 8: 121. DOI: 10.3389/fphar.2017.00121.

Castrignanò S., Bortolussi S., Catucci G., Gholami O., Valetti F., Gilardi G., Sadeghi S.J. (2017) Bioelectrochemical profiling of two common polymorphic variants of human FMO3 in presence of graphene oxide. Electrochimica Acta 228: 611-618. DOI: 10.1016/j.electacta.2017.01.131. 

Castrignanò S., Valetti F., Gilardi G., Sadeghi S.J. (2016) Graphene oxide mediated electrochemistry of glucose oxidase on glassy carbon electrodes. Biotechnology and Applied Biochemistry 63 (2): 157-162. DOI: 10.1002/bab.1392.

Minerdi D., Zgrablic I., Castrignanò S., Catucci G., Medana C., Terlizzi M.E., Gribaudo G., Gilardi G., Sadeghi S.J. (2015) Escherichia coli overexpressing a Baeyer-Villiger monoxygenase from Acinetobacter radioresistens become resistant to Imipenem. Antimicrobial Agents and Chemotherapy 60 (1): 64-74. DOI: 10.1128/AAC.01088-15.

Morra S., Valetti F., Sarasso V., Castrignanò S., Sadeghi S.J., Gilardi G. (2015) Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO₂ adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens. Bioelectrochemistry 106 (B): 258-262. DOI: 10.1016/j.bioelechem.2015.08.001.

Rua F., Sadeghi S.J., Castrignanò S., Valetti F., Gilardi G. (2015) Electrochemistry of Canis familiaris cytochrome P450 2D15 with gold nanoparticles: an alternative to animal testing in drug discovery. Bioelectrochemistry 105: 110-116. DOI: 10.1016/j.bioelechem.2015.03.012.

Castrignanò S., Gilardi G., Sadeghi S.J. (2015) Human flavin-containing monooxygenase 3 on graphene oxide for drug metabolism screening. Analytical Chemistry 87 (5): 2974–2980. DOI: 10.1021/ac504535y.

Di Nardo G.*, Castrignanò S.*, Sadeghi S.J., Baravalle R., Gilardi G. (2015) Bioelectrochemistry as a tool for the study of aromatization of steroids by human aromatase. Electrochemistry Communications 52: 25-28. DOI: 10.1016/j.elecom.2015.01.007. *GD and SC equally contributed to the work.

Minerdi D., Sadeghi S.J., Di Nardo G., Rua F., Castrignanò S., Allegra P., Gilardi G. (2015) CYP116B5: a new class VII catalytically self-sufficient cytochrome P450 from Acinetobacter radioresistens that enables growth on alkanes. Molecular Microbiology 95 (3): 539-554. DOI: 10.1111/mmi.12883.

Castrignanò S., Ortolani A., Sadeghi S.J., Di Nardo G., Allegra P., Gilardi G. (2014) Electrochemical detection of human Cytochrome P450 2A6 inhibition: a step towards reducing dependence from smoking. Analytical Chemistry 86 (5): 2760-2766. DOI: 10.1021/ac4041839.

Di Nardo G., Breitner M., Sadeghi S.J., Castrignanò S., Mei G., Di Venere A., Nicolai E., Allegra P., Gilardi G. (2013) Dynamics and flexibility of human aromatase probed by FTIR and time resolved fluorescence spectroscopy. Plos one 8 (12): e82118. DOI: 10.1371/journal.pone.0082118.

Castrignanò S., Sadeghi S.J., Gilardi G. (2012) Entrapment of human flavin-containing monooxygenase 3 in the presence of gold nanoparticles: TEM, FTIR and electrocatalysis. Biochimica et Biophysica Acta - General Subjects 1820 (12): 2072-2078. DOI: 10.1016/j.bbagen.2012.09.017.

Rua F., Sadeghi S.J., Castrignanò S., Di Nardo G., Gilardi G. (2012) Engineering Macaca fascicularis cytochrome P450 2C20 to reduce animal testing for new drugs. Journal of Inorganic Biochemistry. 117: 277-284. DOI: 10.1016/j.jinorgbio.2012.05.017.

Castrignanò S., Sadeghi S.J., Gilardi G. (2010) Electro-catalysis by immobilised human flavin-containing monooxygenase isoform 3 (hFMO3). Analytical and Bioanalytical Chemistry 398(3): 1403-1409. DOI: 10.1007/s00216-010-4014-z.

Trabace L., Cassano T., Colaianna M., Castrignanò S., Giustino A., Amoroso S., Steardo L., Cuomo V. (2007) Neurochemical and neurobehavioral effects of ganstigmine (CHF2819), a novel acetylcholinesterase inhibitor, in rat prefrontal cortex: an in vivo study. Pharmacological Research 56: 288-294. DOI: 10.1016/j.phrs.2007.07.006.

Trabace L., Kendrick K.M., Castrignanò S., Colaianna M., De Giorgi A., Schiavone S., Lanni C., Cuomo V., Govoni S. (2007) Soluble amyloid beta1-42 reduces dopamine levels in rat prefrontal cortex: relationship to nitric oxide. Neuroscience 147: 652-663. DOI: 10.1016/j.neuroscience.2007.04.056.

Research topics

Studio bioelettrochimico di citocromi P450 umani e flavine monossigenasi su elettrodi di oro e carbone vetroso mediante immobilizzazione in matrici multifunzionali, amplificazione dell’interazione elettrochimica tramite nano particelle d’oro stabilizzate e/o funzionalizzate e ossido di grafene, caratterizzazione spettrofotometrica delle interazioni proteiche tramite FT-IR.

Research groups

• Drug metabolising Enzymes
• Redox chips: Protein Immobilisation for Biosensing

• Enzimi del metabolismo dei farmaci
• Redox chips: immobilizzazione di proteine per biosensori

Academic bodies

• Consiglio di Dipartimento